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primary antibody dilutions  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology primary antibody dilutions
    Primary Antibody Dilutions, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 6136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibody+dilutions/Tubulin+Antibody/pm40854502-145-0-8
    Average 96 stars, based on 6136 article reviews
    primary antibody dilutions - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Membrane:

    Article Title: Rapid Characterization and Quantification of Extracellular Vesicles by Fluorescence‐Based Microfluidic Diffusion Sizing
    Article Snippet: Proteins were transferred on a PVDF membrane (Biorad) with a Mini Trans‐Blot module (Biorad). .. The membrane was blocked with blocking buffer (5% BSA in TBS with 0.1% Tween‐20, TBS‐T) for 2 h at room temperature and incubated overnight at 4 °C under agitation with 1:1000 primary antibody dilutions (CD63 antibody Mx‐49.129.5, CD81 antibody 5A6, Alix antibody 1A12, TSG101 antibody C‐2, Calnexin antibody AF18, GAPDH antibody G‐9, Santa Cruz Biotechnology Inc.) in blocking buffer. .. The membrane was washed four times with TBS‐T and incubated for 2 h at room temperature with a 1:1000 secondary anti‐mouse HRP conjugated antibody dilution (m‐IgG κ BP‐HRP, Santa Cruz Biotechnology).

    Blocking Assay:

    Article Title: Rapid Characterization and Quantification of Extracellular Vesicles by Fluorescence‐Based Microfluidic Diffusion Sizing
    Article Snippet: Proteins were transferred on a PVDF membrane (Biorad) with a Mini Trans‐Blot module (Biorad). .. The membrane was blocked with blocking buffer (5% BSA in TBS with 0.1% Tween‐20, TBS‐T) for 2 h at room temperature and incubated overnight at 4 °C under agitation with 1:1000 primary antibody dilutions (CD63 antibody Mx‐49.129.5, CD81 antibody 5A6, Alix antibody 1A12, TSG101 antibody C‐2, Calnexin antibody AF18, GAPDH antibody G‐9, Santa Cruz Biotechnology Inc.) in blocking buffer. .. The membrane was washed four times with TBS‐T and incubated for 2 h at room temperature with a 1:1000 secondary anti‐mouse HRP conjugated antibody dilution (m‐IgG κ BP‐HRP, Santa Cruz Biotechnology).

    Incubation:

    Article Title: Rapid Characterization and Quantification of Extracellular Vesicles by Fluorescence‐Based Microfluidic Diffusion Sizing
    Article Snippet: Proteins were transferred on a PVDF membrane (Biorad) with a Mini Trans‐Blot module (Biorad). .. The membrane was blocked with blocking buffer (5% BSA in TBS with 0.1% Tween‐20, TBS‐T) for 2 h at room temperature and incubated overnight at 4 °C under agitation with 1:1000 primary antibody dilutions (CD63 antibody Mx‐49.129.5, CD81 antibody 5A6, Alix antibody 1A12, TSG101 antibody C‐2, Calnexin antibody AF18, GAPDH antibody G‐9, Santa Cruz Biotechnology Inc.) in blocking buffer. .. The membrane was washed four times with TBS‐T and incubated for 2 h at room temperature with a 1:1000 secondary anti‐mouse HRP conjugated antibody dilution (m‐IgG κ BP‐HRP, Santa Cruz Biotechnology).

    other:

    Article Title: Pristimerin Contributes to Gefitinib Resistance in Lung Cancer Cells by regulating microRNA-936 expression
    Article Snippet: Related protein expressions were detected by Western blot: HCC827/GR cells in logarithmic growth phase of each group were collected and total cellular protein was extracted, protein concentration was determined by Bicinchoninic Acid (BCA) assay, protein samples were taken for Sodium Dodecyl-Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE), membrane shifted, blocked, CyclinD1 (dilution 1:800), Matrix Metalloproteinase (MMP)-2 (dilution 1:1000), MMP-9 (dilution 1:800), p21 (dilution 1:800) primary antibody dilutions (Santa Cruz, USA), incubated at 24° for 24 h, washed with Tris Buffered Saline Tween® 20 Detergent (TBST) and secondary antibody dilutions (dilution 1:2000) were added (Abcam, USA), Electrochemiluminescence (ECL) was added drop wise and quantity one software was applied to detect the band gray value.



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    The N- and C-termini dependency for TCTN1 in the transition zone localization (A) <t>Immunostaining</t> images showing the ciliary base signals using HA antibody (green) in WT, tctn1 , TCTN1, DUF1619, ΔDUF1619, N-DUF1619, and DUF1619-C cell lines. The signal of acetylated α-tubulin (Ac-tubulin, red) marks the cilium. The white arrowheads mark the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone puncta. The statistical analysis of the percentage of fluorescence localization at the TZ is also presented ( n = 17, 19, 25, 35, 26, 41, and 41). Scale bars, 5 μm.
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    The N- and C-termini dependency for TCTN1 in the transition zone localization (A) <t>Immunostaining</t> images showing the ciliary base signals using HA antibody (green) in WT, tctn1 , TCTN1, DUF1619, ΔDUF1619, N-DUF1619, and DUF1619-C cell lines. The signal of acetylated α-tubulin (Ac-tubulin, red) marks the cilium. The white arrowheads mark the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone puncta. The statistical analysis of the percentage of fluorescence localization at the TZ is also presented ( n = 17, 19, 25, 35, 26, 41, and 41). Scale bars, 5 μm.
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    The N- and C-termini dependency for TCTN1 in the transition zone localization (A) <t>Immunostaining</t> images showing the ciliary base signals using HA antibody (green) in WT, tctn1 , TCTN1, DUF1619, ΔDUF1619, N-DUF1619, and DUF1619-C cell lines. The signal of acetylated α-tubulin (Ac-tubulin, red) marks the cilium. The white arrowheads mark the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone puncta. The statistical analysis of the percentage of fluorescence localization at the TZ is also presented ( n = 17, 19, 25, 35, 26, 41, and 41). Scale bars, 5 μm.
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    Image Search Results


    The N- and C-termini dependency for TCTN1 in the transition zone localization (A) Immunostaining images showing the ciliary base signals using HA antibody (green) in WT, tctn1 , TCTN1, DUF1619, ΔDUF1619, N-DUF1619, and DUF1619-C cell lines. The signal of acetylated α-tubulin (Ac-tubulin, red) marks the cilium. The white arrowheads mark the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone puncta. The statistical analysis of the percentage of fluorescence localization at the TZ is also presented ( n = 17, 19, 25, 35, 26, 41, and 41). Scale bars, 5 μm.

    Journal: iScience

    Article Title: Profiling truncated variants of TCTN1 unveils the essential role of its integrity for ciliogenesis

    doi: 10.1016/j.isci.2025.114190

    Figure Lengend Snippet: The N- and C-termini dependency for TCTN1 in the transition zone localization (A) Immunostaining images showing the ciliary base signals using HA antibody (green) in WT, tctn1 , TCTN1, DUF1619, ΔDUF1619, N-DUF1619, and DUF1619-C cell lines. The signal of acetylated α-tubulin (Ac-tubulin, red) marks the cilium. The white arrowheads mark the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone puncta. The statistical analysis of the percentage of fluorescence localization at the TZ is also presented ( n = 17, 19, 25, 35, 26, 41, and 41). Scale bars, 5 μm.

    Article Snippet: Immunostaining Blocking/Primary Antibody Dilution Solution , Sangon Biotech , Cat#E674004.

    Techniques: Immunostaining, Fluorescence

    The transition zone localizations of NPHP4 or NPHP8 in truncated TCTN1 mutants are not altered (A and B) Immunostaining images displaying the ciliary base puncta of NPHP4 (A) or NPHP8 (B) in WT, tctn1 ::TCTN1-HA (TCTN1), and tctn1 ::TCTN1(DUF1619)-HA (DUF1619) cells. All the cells were immunostained with anti-NPHP4 (green) or anti-NPHP8 (green) and anti-acetylated α-tubulin (Ac-tubulin, red) antibodies. The nuclei were stained with DAPI (blue). The white arrowheads indicate the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone region. The brightfield (BF) and merge channels are also showed. Scale bars, 5 μm. See also for other truncated strains.

    Journal: iScience

    Article Title: Profiling truncated variants of TCTN1 unveils the essential role of its integrity for ciliogenesis

    doi: 10.1016/j.isci.2025.114190

    Figure Lengend Snippet: The transition zone localizations of NPHP4 or NPHP8 in truncated TCTN1 mutants are not altered (A and B) Immunostaining images displaying the ciliary base puncta of NPHP4 (A) or NPHP8 (B) in WT, tctn1 ::TCTN1-HA (TCTN1), and tctn1 ::TCTN1(DUF1619)-HA (DUF1619) cells. All the cells were immunostained with anti-NPHP4 (green) or anti-NPHP8 (green) and anti-acetylated α-tubulin (Ac-tubulin, red) antibodies. The nuclei were stained with DAPI (blue). The white arrowheads indicate the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone region. The brightfield (BF) and merge channels are also showed. Scale bars, 5 μm. See also for other truncated strains.

    Article Snippet: Immunostaining Blocking/Primary Antibody Dilution Solution , Sangon Biotech , Cat#E674004.

    Techniques: Immunostaining, Staining